Engineered ribosomes with tethered subunits for expanding biological function
نویسندگان
چکیده
منابع مشابه
Mass spectrometry of ribosomes and ribosomal subunits.
Nanoflow electrospray ionization has been used to introduce intact Escherichia coli ribosomes into the ion source of a mass spectrometer. Mass spectra of remarkable quality result from a partial, but selective, dissociation of the particles within the mass spectrometer. Peaks in the spectra have been assigned to individual ribosomal proteins and to noncovalent complexes of up to five component ...
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Wo have developed a method for preparation and purification of active ribosomal subunits from rat and mouse liver. Our technique involvea incubating purified polysomes with all components for protein synthesis until all competent ribosomes have terminated and released their polypeptide chain. Upon kreatment of such an incubation mixture with 0.5 M-KCl, followed by sucrose gradient centrifugatio...
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ژورنال
عنوان ژورنال: Nature Communications
سال: 2019
ISSN: 2041-1723
DOI: 10.1038/s41467-019-11427-y